Posted 17 October 2001 - 09:00 PM
Posted 21 October 2001 - 09:00 PM
A good way to find out is by simply trying. Ofcourse this is RNA you're working with and I only have experience with DNA electrophoresis. With that, 100 Volts for 1 hour or so is very good useable. I think a somewhat higher Voltage would be better, including things like a less dense gell.
Posted 23 October 2001 - 09:00 PM
I personally do not believe that altering the voltage at which you run it will result in more concise bands.I would first spread the sample out into a few wells. what's the prob. with such wide bands. are you extractingfrom the gel?