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Using Biorad SDS-PAGE apparatus for CDGE


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#1 Rethonji

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Posted 22 March 2019 - 05:44 AM

I am planning to use CDGE in one of my experiments. However, currently we dont have a DCODE Biorad for DGGE experiments. Based on the apparatus and the method for CDGE, it looks like I can employ the method using SDS-PAGE system. Is it possible? Has anyone tried this?



#2 iosman987

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Posted 01 May 2020 - 10:00 AM

I need 10 mM stock solution of dNTP. So I'm assuming you just combine all four at equal amounts to create a 100 mM solution of dNTP. Then you dilute 1/10 to create a 10 mM stock of dNTP which will be suitable for PCR? Never done it this way, and I'm 99% sure this will work, however in Science, even the little things get you. Thank you



#3 iosman987

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Posted 07 May 2020 - 04:30 AM

I need 10 mM stock solution of dNTP. So I'm assuming you just combine all four at equal amounts to create a 100 mM solution of dNTP. Then you dilute 1/10 to create a 10 mM stock of dNTP which will be suitable for PCR? Never done it this way, and I'm 99% sure this will work, however in Science, even the little things get you. Thank you

 

thanks my issue has been fixed.

 






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