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Isotype control vs. stained control

isotype control flow cytometry

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#1 Thomson



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Posted 14 March 2019 - 06:59 AM



For flow cytometry I used stained negative control (cell not expressing the surface protein but are stained with antibodies similar to sample) and there are no background noticed for all 3 flurochromes used. I used it to gate against the signals. Like below 10^2 is negative signal, above 10^2 is positive signal. 


I thought it should be sufficient. Is isotype control necessary?

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