4 replies to this topic

[Fred-33]

I need to know if a human macrophage cell line exists at time.
Many thanks for help.

[Fred-33]

I found my answer on atcc site

here are some useful lines from spleen ant monocytes/macrophages
HB-8902
CRL-9850
CRL-9852
CRL-9853
CRL-9854
CRL-9855
CRL-9856

[serglom]

Fred-33, on Sep 8 2004, 07:44 AM, said:

I found my answer on atcc site

here are some useful lines from spleen ant monocytes/macrophages
HB-8902
CRL-9850
CRL-9852
CRL-9853
CRL-9854
CRL-9855
CRL-9856

you can also use THP-1 monocyte cell line and turn them into macrophages with TPA or PMA

[emilydeanna]

I have just started culturing THP-1 cells.

After sub-culturing, I wanted to freeze down some of these cells. I froze a vial of cells with my normal freezing media (2x, 20% DMSO and 80% FBS).  When I thawed the cells they were viable.  After 48h the cells had switched from being in suspension to becoming adherent.

Is this from the DMSO?

Should I use glycerol to cryopreserve instead?

[SuMi]

emilydeanna, on Jan 28 2009, 11:01 PM, said:

I have just started culturing THP-1 cells.

After sub-culturing, I wanted to freeze down some of these cells. I froze a vial of cells with my normal freezing media (2x, 20% DMSO and 80% FBS).  When I thawed the cells they were viable.  After 48h the cells had switched from being in suspension to becoming adherent.

Is this from the DMSO?

Should I use glycerol to cryopreserve instead?

Yes I have found that DMSO causes my cells to differentiate. 20% DMSO is very high - 10% is enough. I now use a special freezing medium for my cells as i had a huge problem with differentiation. When you thaw your cells you should spin them down at a low speed and remove the freezing medium to remove the DMSO.