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Human Macrophage cell line - quick help please


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4 replies to this topic

#1 Fred-33

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Posted 08 September 2004 - 03:38 AM

:D I need to know if a human macrophage cell line exists at time.
Many thanks for help.

#2 Fred-33

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Posted 08 September 2004 - 06:44 AM

I found my answer on atcc site

here are some useful lines from spleen ant monocytes/macrophages
HB-8902
CRL-9850
CRL-9852
CRL-9853
CRL-9854
CRL-9855
CRL-9856

#3 serglom

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Posted 14 November 2004 - 02:03 PM

I found my answer on atcc site

here are some useful lines from spleen ant monocytes/macrophages
HB-8902
CRL-9850
CRL-9852
CRL-9853
CRL-9854
CRL-9855
CRL-9856

you can also use THP-1 monocyte cell line and turn them into macrophages with TPA or PMA

#4 emilydeanna

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Posted 28 January 2009 - 02:01 PM

I have just started culturing THP-1 cells.

After sub-culturing, I wanted to freeze down some of these cells. I froze a vial of cells with my normal freezing media (2x, 20% DMSO and 80% FBS). When I thawed the cells they were viable. After 48h the cells had switched from being in suspension to becoming adherent.

Is this from the DMSO?

Should I use glycerol to cryopreserve instead?

#5 SuMi

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Posted 03 February 2009 - 06:58 AM

I have just started culturing THP-1 cells.

After sub-culturing, I wanted to freeze down some of these cells. I froze a vial of cells with my normal freezing media (2x, 20% DMSO and 80% FBS). When I thawed the cells they were viable. After 48h the cells had switched from being in suspension to becoming adherent.

Is this from the DMSO?

Should I use glycerol to cryopreserve instead?


Yes I have found that DMSO causes my cells to differentiate. 20% DMSO is very high - 10% is enough. I now use a special freezing medium for my cells as i had a huge problem with differentiation. When you thaw your cells you should spin them down at a low speed and remove the freezing medium to remove the DMSO.




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