Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

problem on membrane preparation


  • Please log in to reply
1 reply to this topic

#1 lilian_gz

lilian_gz

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 07 September 2004 - 10:19 AM

I followed the protocol in our lab to prepare cell membrane from mice tissue. But I got membrane much less than what they did before. We discussed over but it seems the only reason will be the insufficient homogenization. However, I really think I chopped the tissue small enough, so I'm wondering what will be the key to reduce the yield of the membrane because I really don't understand very well about the protocol: why we can get membrane at this speed of centrifugation?

Is there anyone have a good idea of this?

#2 Carolius

Carolius

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 September 2004 - 10:41 PM

Hi Lilian

In our lab we have experience in tissue homogenization and membrane purification, but given that the different protocols are slightly different, would be fine that you indicate the protocol that you apply for mice.

Carolius




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.