Protein purification questions
Posted 27 February 2019 - 02:00 PM
2) What is the protocol for batch purification to remove TEV protease via the use of NI-NTA agarose beads?
Posted 27 February 2019 - 02:29 PM
1) Weight ratios mean exactly that a ratio based on weight (mass) - if you have 1 mg of substrate, add 3 mg of TEV.
2) I suggest you google: on-column protease digestion his-tag
Posted 28 February 2019 - 12:57 AM
Posted 28 February 2019 - 09:18 AM
Sounds about right. I see in my post above I got the ratios around the wrong way, so you even managed to interpret what I intended correctly.