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Guidelines for autoclaving

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#1 lucast



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Posted 22 February 2019 - 03:50 AM

Guidelines for autoclaving
While every Lab follows its own set of autoclaving guidelines, the following recommended steps must be kept in mind.
Preparation of autoclave and materials: All Science Equipment that needs to go in the autoclave should be washed with distilled water beforehand. Only materials that are safe and can withstand autoclave steam and heat pressure should be selected. If necessary, stainless steel or polypropylene tubs should be used as separate containment with enough space for steam circulation.
Sealing: Although autoclaves lock automatically, manual locks must be safely secured to prevent accidental leakage.
Selection of cycle and start: The recommended cycle should be chosen as per goods, for instance, liquid or slow exhaust cycle is for dry equipment like lab glassware. The specific sterilization and drying time should be set and start pressed.
End Safety: Using protective gloves, allowing items to cool before touching and letting pressure return to zero before opening are essential safety measures to prevent accidents.

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