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Help, how to perfuse mouse, remove blood, and harvest blood-free thymus?

thymus perfusion organ harvest blood

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#1 yl2j

yl2j

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Posted 20 February 2019 - 06:37 PM

Hello everyone,
 
I need help getting rid of blood in mouse and harvest thymus.
 
I intend to harvest thymus without blood in it or on it.
 
I've tried the traditional way of pumping liquid into one chamber of the heart while letting blood drain out of another chamber of the heart. As I do so, the blood draining out starts to pool within the thoracic cavity. Since the thymus is right on top of the heart, almost every time the blood gets on the thymus.
 
I need to know what's another blood vessel location that comes after the thymic circulation where I can cut that section of the vessel and so the thymus I harvest would not get any blood on it.
 
I need to blood circulation map of the mouse, particularly in relation to the thymus.
 
Many thanks!
 
Yong

 


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