Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

Immunofluorescence quantification

Started by biodena86, Jan 24 2019 08:31 AM

2 replies to this topic

member

0
Neutral

Posted 24 January 2019 - 08:31 AM

Hi,
I did immunohistochemistry for parvalbuminergic neurons and their perineuronal matrix. What kind of programm do you suggest to quantify them?

Thelymitra pulchella

565
Excellent

Posted 24 January 2019 - 09:28 AM

Fiji, which is an implementation of ImageJ designed and pre-packaged for image analysis.

Veteran

61
Excellent

Posted 19 March 2020 - 09:11 AM

If you have no internal reference, it would be semi-quantitative only

Protocols and Techniques Forums → General Lab Techniques → Standard curve using ImageJ Started by CristianaCris, 14 Jun 2016 strandard curve, dna amount and 1 more...	4 replies 1,444 views	CristianaCris 08 Jul 2016
Protocols and Techniques Forums → Protein and Proteomics → Quantifying bands on PAGE gel with ImageJ Started by Michael Starr, 24 Feb 2016 ImageJ, PAGE, debugging and 1 more...	0 replies 2,117 views	Michael Starr 24 Feb 2016
Protocols and Techniques Forums → Bioinformatics and Biostatistics → which statistical test should i use Started by BMF, 30 Aug 2012 statistical test, correlation and 2 more...	7 replies 1,541 views	bob1 01 Sep 2012
Protocols and Techniques Forums → General Lab Techniques → Is it possible to save your progress counting in ImageJ? Started by assembler01, 10 Apr 2012 imagej, save, count	0 replies 1,570 views	assembler01 10 Apr 2012