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Immunofluorescence quantification

Started by biodena86, Jan 24 2019 08:31 AM

2 replies to this topic

member

0
Neutral

Posted 24 January 2019 - 08:31 AM

Hi,
I did immunohistochemistry for parvalbuminergic neurons and their perineuronal matrix. What kind of programm do you suggest to quantify them?

Thelymitra pulchella

565
Excellent

Posted 24 January 2019 - 09:28 AM

Fiji, which is an implementation of ImageJ designed and pre-packaged for image analysis.

Veteran

61
Excellent

Posted 19 March 2020 - 09:11 AM

If you have no internal reference, it would be semi-quantitative only

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