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PBS or TBS, where cannot use each buffers?


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#1 koreanraichu

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Posted 21 January 2019 - 06:16 PM

Greetings.
 
As you know, Tris buffered saline and Phosphate buffered saline is multipurpose. For finding each buffer's use, there's so many use experiment for PBS and TBS. TBS uses for western blotting, and PBS uses for cell culture.
 
but some experiment never use PBS and some experiment never use TBS, I want to ask this situation which when you use TBE but never use PBS, and the opposite situation(use TBS but never use PBS), For example, When you western-blotting with alkaline phosphatase never use PBS and PBS-T. this case, phosphate inhibits AP, and amine-coupling no use Tris because it have primary amine.


#2 mdfenko

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Posted 23 January 2019 - 03:03 PM

you also don't want to use pbs when you use calcium (or any other cation that forms an insoluble product when combined with phosphate)


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