I have a question but there's no answer about this...
In SDS-PAGE, uses Tris-glycine buffer w/ SDS for gel loading buffer. SDS set a charge of protein for negative and Tris is buffering agent, Glycine helps protein move and separation during electrophoresis.
I don't know where Tris-glycine buffer uses and what's different. I thought buying buffer without SDS and add SDS.
Is there situation unfit for use SDS during protein electrophoresis?