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why Tris-glycine buffer divided by w/ SDS and w/o SDS?


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#1 koreanraichu

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Posted 20 January 2019 - 08:32 AM

Hello.

 

I have a question but there's no answer about this...

In SDS-PAGE, uses Tris-glycine buffer w/ SDS for gel loading buffer. SDS set a charge of protein for negative and Tris is buffering agent, Glycine helps protein move and separation during electrophoresis.

I don't know where Tris-glycine buffer uses and what's different. I thought buying buffer without SDS and add SDS.

 

Is there situation unfit for use SDS during protein electrophoresis?



#2 bob1

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Posted 20 January 2019 - 06:25 PM

The without is usually used in the transfer process, and sometimes for native (non-denaturing) gels.






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