I recently got one of my review articles rejected. One of the reviewers made some comments about a paragraph that I had. Do you agree with him? By practice we have found that if we use hich copy plasmids for recovery of virus then more cells die. The reviewer says that this does not depend on the copy number, but the promoter. We used the same T7 promoter for both plasmids. The low copy ones were less toxic.
Paragraph 2.1 contains a number of statements that are not correct. The authors argue that the use of high-copy number vectors has "... the advantage that they result in high transcription rate". This is not true; the transcription rate in eukaryotic cells is primarily determined by the promoter, not by the plasmid copy number in E.coli. Also the statement that "... low-copy number transcription vectors usually produce less toxicity in cell culture compared to high-copy number vectors ..." is not true. The choice between a low- or high-copy number plasmid is primarily one of convenience (higher yield) or plasmid stability in E.coli.