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Monocyte maturation


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#1 chloeo

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Posted 03 September 2004 - 02:04 AM

HI
Can anyone suggest or offer advise on suitable markers for monocytes, macrophages and dendritic cells. I need to distinguish between the three cell types. I was thinking of using CD156a for monocytes, CD169 for macrophages and CD1a/DC LAMP for dentritic cells. Any ideas, comments would be most appreciated.
Thank-you

#2 immune platelet

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Posted 16 September 2004 - 08:23 AM

For DC, you can use CD205 (DEC205). You can also use CD11c. For macrophages we typically use F4/80. If I remember correctly, you can double stain with CD14 and F4/80 for monocytes (hi CD14) and macrophages (low CD14). There is a great deal published in the area and you may want to do a PubMed search. The markers you chose will only identify subsets of each cell type.




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