for the end of my high school biology assessment, I decided to investigate the effect concentration of sulphuric acid in acid rain has on the concentration of pigments (primarily chlorophyll) in Sphagnum moss. Having five samples, each being watered with differently concentrated acid, I carried out photospectrometry for absorption (using ethanol as a solvent) for wavelengths 750nm, 700nm, 675nm, 665nm, 645nm, 630nm, 570nm and 540nm. My prediction was that more acidic environment will be, less pigment will be present (chlorophyll will fall apart).
Is this prediction correct? How will it influence the absorption rate of the samples? Can the concentration/amount of individual pigments be calculated based on photospectrometry? How can these samples be compared? What statistical test can be used to show whether the data are valid?
Thank you for your answer.