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Mammalian two-hybrid?

Two-Hybrid Assays Luciferase assay protein interaction missense mutation

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#1 Lauretta77

Lauretta77

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Posted 18 December 2018 - 03:01 AM

Hi, 
I have to evaluate and accurately quantify the effect of missense mutations on binding between two known interacting proteins.
To this end, is it better measure SEAP protein (Matchmaker Mammalian Two-Hybrid assay kit and Great EscAPe SEAP Chemiluminescence Detection Kit, clontech) or Luciferase assay?
 
Thank you in advance
Sincerely,
 
Laura






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