Hi all, I am trying to cultivate bovine respiratory syncytial virus (BRSV) on MDBK cells but my TCID50 is still low (around 5.6 no more). I need to cultivate it in high titre (ideally around 8.0).
Do you have any idea how to do this? I´ve tried a lot of things - different time of collection, different cultivation temperature, different composition of growing medium - but the result was always the same - not so high titre.
I have also found a references in the literature that mention that most BRSV viruses remain associated with sycytial cells. This could be the cause of a low virus titer in the collection. Please, does anyone have experience with high titre BRSV cultivation?
Edited by Scientist1, 12 December 2018 - 05:30 AM.