i am currently looking for IL-36 in the Hacat cell line.
I've been trying a month but I have no a good result TT.
my protocol is
- Blocking with 5% BSA
- 1st Ab at 1:500 (goat-anti IL36) at 4 degree overnight (I also tried 1:1000)
- 2st Rabbit anti goat (1:1000) at RT for 1 hour
PS I use all new buffer and carefully made the buffer and my interested protien is 17 kDa
Please someone give me any suggestion TT
Edited by Vui, 06 December 2018 - 04:31 AM.