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Microisland formation

Started by Mad Researcher, Nov 30 2018 12:55 PM

microisland cell culture primary neurons

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#1 Mad Researcher

Newbie !!

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Posted 30 November 2018 - 12:55 PM

Hi,

I am currently making cortical primary neuronal culture from P0-P2 mice pups.

After about 5-7 days in-vitro, I see microisland formation. What is the cause of the microisland formation? Is it a good or bad thing to have in cell culture? If it is bad, how can I avoid the formation of microislands?

Thanks

Cheers,

Mad Researcher

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