Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

Microisland formation

Started by Mad Researcher, Nov 30 2018 12:55 PM

microisland cell culture primary neurons

Please log in to reply

No replies to this topic

#1 Mad Researcher

Newbie !!

Active Members
255 posts

15
Good

Posted 30 November 2018 - 12:55 PM

Hi,

I am currently making cortical primary neuronal culture from P0-P2 mice pups.

After about 5-7 days in-vitro, I see microisland formation. What is the cause of the microisland formation? Is it a good or bad thing to have in cell culture? If it is bad, how can I avoid the formation of microislands?

Thanks

Cheers,

Mad Researcher

Back to Tissue and Cell Culture

Also tagged with one or more of these keywords: microisland, cell culture, primary neurons

Protocols and Techniques Forums → Tissue and Cell Culture → MNFS60 cells are dying. Started by biouser, 23 Jan 2019 cell culture, MNFS60 cells and 1 more...	2 replies 2,357 views	Stephbaker 25 May 2019
Protocols and Techniques Forums → General Lab Techniques → Help Needed Finding Some Plasmid Prep Protocols Started by Gandalf99, 28 May 2018 plasmid, cell culture and 2 more...	2 replies 4,237 views	bob1 03 Jul 2018
Protocols and Techniques Forums → Tissue and Cell Culture → Strange contamination issue Started by mia123, 08 May 2018 contamination, cell culture	4 replies 1,090 views	mia123 08 May 2018
Protocols and Techniques Forums → Cell Biology → Should Lineage marker cocktail be used in cell lines to remove lineage specific Started by rv1234, 18 Apr 2018 Cell Culture, FACS, CellLines and 1 more...	1 reply 2,074 views	Creative Biolabs 19 Jun 2018
Protocols and Techniques Forums → General Lab Techniques → Beads coated with integrins? Started by EvaG, 28 Mar 2018 integrin, biotinylation, beads and 2 more...	1 reply 1,666 views	bob1 04 Apr 2018