Hi all, I often get two populations from my results of electroporated cells compared to non-electroporated control (pic attached). Left is non-electroporated,, right is electroporated.
3 million cells, 5ug plasmid, exponential (250V, 950 uF, Ω infinite , cuvette 4mm ).
What could be the reason?
I don't think it's doublet as the fluoresence intensity of both population almost similar when measure individually.
Edited by Thomson, 28 November 2018 - 04:23 AM.