I hope you will hear from someone who has actually tried this. I have not, and I generally advise against using components from different branded kits in the same prep. However, the two lysis buffers both contain salts of guanidine (complete compositions are proprietary) and you add beta-mercaptoethanol to both, so they are similar. Since you have already done the lysis, I’d try processing just 1 or 2 (for balance) through the Qiagen system and see what you get. If it doesn’t work, and the rest of the samples are hard to replace, get the kit that matches the lysis buffer to finish them. Maybe you can borrow some from another lab? Good luck! By the way, if you tried it and it did work, report it here for the next person who needs to know
Edited by OldCloner, 21 November 2018 - 06:28 AM.