Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Please help me determine if this math for creating a stock solution and drug tre

cisplatin drug concentration mg/mL lab math

  • Please log in to reply
2 replies to this topic

#1 mugjay

mugjay

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 07 November 2018 - 08:37 AM

Hi All, 

 

I am new in my lab and having trouble figuring out how to create a stock solution of powdered Cisplatin (1g in a bottle).  And from there, determining how much of the stock I must add to media in order to treat my cells. 

 

The molecular weight of cisplatin is 300.01 g/mol. I attempted to do some of the math myself. 

 

I know that a 10mM concentration can be made using 3mg/mL. By multiplying by 1000 (to scale down to uM), I end up with 10,000 uM = 3000 mg/1000mL. I need a final concentration of 30uM, so I divided by 333.33 to end up with 30uM = 9mg/3mL. 

 

 

For the cell treatment, I need a concentration of 30 uM. Does this mean that I can add 27 uL of the 30 uM stock to 9mL of media?

 

Did I approach this math correctly? 



#2 mdfenko

mdfenko

    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,292 posts
202
Excellent

Posted 07 November 2018 - 11:08 AM

do you want 30 uM in the medium (ie final concentration)? if so, then you can't use a stock which is already at that concentration.

 

using the 10 mM stock, use the formula v1c1=v2c2 (v=volume, c=concentration) and rearrange so that you can determine how much of the stock to use: v1=(v2c2)/c1. make sure you are consistent with the units.

 

v1=(9000 ul x .03 mM)/10 mM=27 ul of the 10 mM stock

 

try not to overcomplicate (or overthink) the problem


talent does what it can
genius does what it must
i do what i get paid to do

#3 mugjay

mugjay

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 November 2018 - 01:34 PM

Thank you so much for your help! You are right, I was overthinking.







Also tagged with one or more of these keywords: cisplatin, drug concentration, mg/mL, lab math

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.