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Fixation for surface epitope detection

flow cytometery surface marker fixation

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#1 Thomson

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Posted 05 November 2018 - 04:45 PM

Hi,

 

Is it advisable to not fix the cells after extracellular staining for surface marker using flow cyto?

 

I mean, if I will directly go for detection after staining of the samples. Not planning to store for long use.


Edited by Thomson, 05 November 2018 - 04:45 PM.


#2 mdfenko

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Posted 07 November 2018 - 11:10 AM

i have no experience with flow cytometry but it would seem to me that you would not want to use fixed cells for the process.


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