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Fixation for surface epitope detection

flow cytometery surface marker fixation

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#1 Thomson



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Posted 05 November 2018 - 04:45 PM



Is it advisable to not fix the cells after extracellular staining for surface marker using flow cyto?


I mean, if I will directly go for detection after staining of the samples. Not planning to store for long use.

Edited by Thomson, 05 November 2018 - 04:45 PM.

#2 mdfenko


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Posted 07 November 2018 - 11:10 AM

i have no experience with flow cytometry but it would seem to me that you would not want to use fixed cells for the process.

talent does what it can
genius does what it must
i used to do what i got paid to do

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