Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

black spots are found in cells and medium

  • Please log in to reply
1 reply to this topic

#1 hzaubl



  • Members
  • Pip
  • 1 posts

Posted 31 October 2018 - 11:50 PM

 recently, i find there are a lot of black spots in cell and medium at low-power field (fig.1). at high-power field, the black spots look like Vesicles(fig.2). the medium is M199 with 0.1 FBS, the cell is FHM( fathead minnow), it was cultured at 28 degrees Celsius with 0.5 co2. 

this phenomenon had bad effects on the cell, dose anyone has the same problem or konw what's the matter? 


thanks a lot

Attached Thumbnails

  • 6)JMOC3MTE5(M5W~V$91LZ7.jpg
  • BBC4MX2%5MAF`%OHAQM4D.png

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,740 posts

Posted 01 November 2018 - 05:48 AM

The answer is pinned in the cell culture subforum: http://www.protocol-...-helpful-paper/


Bascially it boils down to that the black dots are bacteria and very hard to get rid of. They most likely come from your waterbaths or possibly from the water pans in your incubators.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.