if anybody has a copy of LiAc transofrmation' protocol from Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor NY (1994, Kaiser et, al.).
I have a problem with transformation - I received a plasmid which overproduce SLA1. I want to transform strains which have deleted genes involved in assembly in the cytoskeleton. In the paper is written that they used transformation protocol from that book but unfortunately I can't find it anywhere .
I tried many protocols but none work. I really want to test protocol from that book.
Please, help me! You are my last hope.
Edited by Tysia91, 26 October 2018 - 11:46 AM.