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LiAc transformation - HELP!

LiAc transformation yeast

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#1 Tysia91

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Posted 26 October 2018 - 11:41 AM

Hi guys, 

 

if anybody has a copy of LiAc transofrmation' protocol from Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor NY (1994, Kaiser et, al.).

 

 

I have a problem with transformation - I received a plasmid  which overproduce SLA1. I want to transform strains which have deleted genes involved in assembly in the cytoskeleton. In the paper is written that they used transformation protocol from that book but unfortunately I can't find it anywhere .

 

I tried many protocols but none work. I really want to test protocol from that book. 

 

Please, help me! You are my last hope.

 

Thank you,

Justyna


Edited by Tysia91, 26 October 2018 - 11:46 AM.


#2 bob1

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Posted 26 October 2018 - 12:25 PM

I can't easily get that one but I can get this one: 

 

Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method: Geitz, R.D. 2014.

from here



#3 Tysia91

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Posted 29 October 2018 - 01:53 AM

Dear Bob1,

 

Thank you for your help, I'm very grateful.

I used this method and didn't work with my plasmid. I realy want to find method from Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor NY (1994, Kaiser et, al.) becouse I want know what they did diffrent to achieve the success.

 

Best reagrds,

Justyna



#4 OldCloner

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Posted 29 October 2018 - 05:08 AM

Hi Justyna,

 

This is a job for your friendly neighborhood Librarian! They can often get access to things the rest of can't, through Interlibrary Loan.  I'd recommend a visit to your University library!

 

Good luck!







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