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Use of CRISPR technology to introduce a gain-of-function mutation

Started by Martin23, Sep 26 2018 04:18 AM

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#1 Martin23

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Posted 26 September 2018 - 04:18 AM

Dear all,

 
I want to use CRISPR technology to introduce a gain-of-function mutation. Has anyone tried to do that, and if so I guess that the selection process is quite difficult.
 
I hope someone can help me.
 
Thank you.
 
Best wishes
Martin

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Posted 27 July 2019 - 11:16 PM

Refer to,

 

https://www.nature.c...cles/nmeth.4076

 

https://www.nature.c...cles/nmeth.3274


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