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Overview of Quantitative Proteomics

Proteomics Quantitative Proteomics

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#1 larry

larry

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Posted 12 September 2018 - 10:27 PM

With the development and maturity of mass spectrometry, the technical advantages of high throughput, high sensitivity and wider dynamic range have been emerged, which provides a reliable research method for proteomics research. In recent years, quantitative proteomics has become one of the research hotspots in omics field.

 

Quantitative proteomics is an emerging technology used for accurate quantification and identification of proteins expression in biological samples. It can not only identify the proteins expressed in different states, but also accurately quantify their abundance. According to different quantitative purposes, quantitative proteomics can be divided into two categories relative quantitative proteomics and absolute quantitative proteomics. Relative quantitative proteomics, also known as comparative proteomics, is a comparative analysis of protein expression level in cells, tissues or organism in different physiological or pathological conditions. Absolute quantitative proteomics is the determination of the absolute quantity or concentration of each protein in a proteome.

 

Four Methods Commonly Used in Quantitative Proteomics Research

 

Label-free

SILAC

iTRAQ 

MRM

 

Applications and Significance of Quantitative Proteomics

 

Quantitative proteomics technology has been widely applied to identify diagnostic expression patterns specific to pathological cellular states, and to provide clues to the genes and gene products that are responsible for, or correlate with, a specific state of a system. It also has been used to accurately quantify human tumor proteomes, and to investigate the mechanism of the antifungal agent and the target of drug action.

 

Quantitative proteomic technology not only is of significant interest in studies aimed at discovering disease biomarkers and providing new insights into biological pathways, but also holds significant promise for the discovery of diagnostic or prognostic protein markers for the detection of new therapeutic targets. 

 

 







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