i want to test the enzyme for characterize antifungal property. but i want to assay on the fresh leaves. i found the paper that explained how to test like this ,they test on the orchid fresh leaves by scoop the leaves then drop the agent in. they did it by did not pick the leaves off.
but my case is difference. the orchid leaves are thick and hard, so they can scoop it. but my leaves ( mulberry leaves ) are fragile.
and how can i contain the fresh of leaves if i pick it off ?
can i use paper disc test on the fresh leaves ?
i want to know how can i assay the enzyme on the fresh leaves, i did not found the paper about this before.