Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

RT-PCR efficiency, error, R2

real time pcr efficiency error r-square

  • Please log in to reply
No replies to this topic

#1 Thomson



  • Active Members
  • PipPipPipPipPip
  • 34 posts

Posted 06 September 2018 - 08:52 PM

Hi all, 


usually for my real time pcr, the efficiency is around 1.87 to 1.95, error is 0.25 to 0.45, R2 is usually 1. 


This time I get efficiency 1.87, R2= 0.91, and error is as high as 1.87. 


Do you guys think the R2 is too low?

And also, what does the error means in this context?


Attached with photo.

Attached Thumbnails

  • Capture.JPG

Also tagged with one or more of these keywords: real time pcr, efficiency, error, r-square

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.