I am reading a paper that can be found in the attachment or here: https://www.cell.com...secsectitle0020
A simple question on this paper =>
Picture 3 C : does the picture seem ok to you?
Am I missing something here or is the left part of the picture rather strange compared to the right part?
They show Co-IP, but in the input sample you do not see a band in the Pkc1 lane which is weird in my opinion.
If it is the same blot then why is there no band? My only idea is that it is because Pkc1 is only present in such low amounts that you need to do an IP first to concentrate it more to be shown on a blot later. However it seems weird this is not mentioned in the paper + in picture 3A you can clearly see a band in the Pkc1 lane, which was a simple "input" sample so not sure why they have no band in picture 3C.
I find this type of pictures/blots rather fishy.
Or am I missing something crucial here? I just can not seem to figure this one out.