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Newborn calf serum used in place of FCS

Calf serum FCS FBS cell cultue

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#1 Strife



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Posted 31 August 2018 - 02:28 PM

Hello to everyone,


I'm new in this group,


I have this question: I cultured a cancer cell line (HCC1143) using a medium with 10% bovine serum, however on ATCC is reported to use 10% of fetal calf serum (FCS). I did it by mistake, however cells grew as normal and I noticed no particular issues, subsequently I performed RNA-seq before I noticed the mistake. Does anyone knows how much this can affect the results?


In your opinin, can I not be worried since cell grew up normally?


As I now switch to FCS for my other cancer cell line, the results could be comparable with this cell line? 


I hope in your suggestion. Thank you in advance.




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