Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

RT-qPCR of Micro RNA host gene

Started by Zara33, Aug 24 2018 10:02 AM

RNA-seq RT-qPCR microRNA

Please log in to reply

No replies to this topic

#1 Zara33

member

Members
1 posts

0
Neutral

Posted 24 August 2018 - 10:02 AM

Hi,

I got a differentially expressed long non-coding RNA by RNA-Seq. It is MIR17HG or the host gene of the Mir 17-92 cluster. I know qPCR of the individual micro RNAs can be done but is it possible to design primers and carry out RT-qPCR on the pri-mirRNA17-92 primary transcript?

Edited by Zara33, 24 August 2018 - 10:02 AM.

Back to PCR, RT-PCR and Real-Time PCR · Next Unread Topic →

Also tagged with one or more of these keywords: RNA-seq, RT-qPCR, microRNA

Products and Vendors Discussion → Molecular Biology Products → Powerful and Intuitive Gene Expression Visualization Tools to Interpret Biologic Started by Partek, 12 Nov 2018 gene expression, visualization and 2 more...	0 replies 819 views	Partek 12 Nov 2018
Protocols and Techniques Forums → siRNA, microRNA and RNAi → lizard microRNA and human lncRNA Started by Fredreload, 21 Mar 2017 microRNA	1 reply 1,592 views	Fredreload 26 Mar 2017
Protocols and Techniques Forums → Molecular Biology → Concatemerization with miRCat cloning kit Started by Correaus microTaenia, 28 May 2016 Concatemerization, miRCat and 2 more...	0 replies 1,702 views	Correaus microTaenia 28 May 2016
Protocols and Techniques Forums → Genetics and Genomics → Enhancer Identification for Specific Genes Started by SarahLee8, 09 Nov 2015 enhancers, qpcr, rna-seq	0 replies 35,824 views	SarahLee8 07 Dec 2015
Protocols and Techniques Forums → PCR, RT-PCR and Real-Time PCR → Calculation of relative gene expression using Livak method Started by awan, 26 Sep 2015 RT-qPCR, Molecular Biology and 1 more...	0 replies 1,373 views	awan 26 Sep 2015