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RT-qPCR of Micro RNA host gene

RNA-seq RT-qPCR microRNA

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#1 Zara33

Zara33

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Posted 24 August 2018 - 10:02 AM

Hi,

 

I got a differentially expressed long non-coding RNA by RNA-Seq. It is MIR17HG or the host gene of the Mir 17-92 cluster. I know qPCR of the individual micro RNAs can be done but is it possible to design primers and carry out RT-qPCR on the pri-mirRNA17-92 primary transcript?


Edited by Zara33, 24 August 2018 - 10:02 AM.






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