Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Multi-staining 7-aad, Annexin-V, Cell Trace CFSE, Dyecycle Violet

Flow cytometry apoptosis multi-stain

  • Please log in to reply
No replies to this topic

#1 single_cell_nerd_88

single_cell_nerd_88

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 02 August 2018 - 11:11 PM

​Hi,

 

​I had a question for the Flow experts here. Has anyone tried staining with multiple dyes - 7-AAD, Annexin-V (PE) - part of Annexin V Apoptosis Detection Kit from BD Pharmingen. These are to be coupled with Dyecycle Violet (BV421) and Cell Trace CFSE (FITC). I have the following problems -

 

1) 7-AAD seems to be bleeding into the PE channel. I've tried compensating and I have been unable to do so

2) Also Annexin V (PE) is bleeding into the Dyecycle channel. 

​3) Cell Trace is bleeding into Annexin V and 7-AAD channels. I've tried knocking them down.

 

I have already tried compensation. Automatic and manual. I am running my samples on the FACS-Canto-II. 

 

​Has anybody else tried this combination or has experienced similar issues?

 

Thanks

 







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.