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Dilution of antibody

antibody dilution

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#1 Rojan D.

Rojan D.


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Posted 31 July 2018 - 01:10 AM

hi everyone. 


im a newbie in the lab and I've ordered a new antibody that recommend to use the diluton 1µg/ml. 

The vial is 100µl of the antibody. However, I do not know the concentration within the 100µl. 


Can anybody help me to resolve my question which is, what is 1µg/ml in terms of dilution factors such as 1:1000 etc. 



Edited by Rojan D., 31 July 2018 - 01:12 AM.

#2 bob1


    Thelymitra pulchella

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Posted 31 July 2018 - 05:49 AM

Check the data sheet that came with the antibody and have a look on the tube too. If you can post a link to the antibody that might help


Antibodies commonly come as 1 mg/ml and 0.5 mg/ml concentrations, though other concentrations are also supplied, so don't assume either of these. 

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