I'm a newbie and I really need help to comprehend and interpret this WB result.
Referring to the attached result, I'm using TRPV4 agonist (GSK101) 100nM concentration.
Then, I used 2 different TRPV4 antagonists, which are HC067 and GSK219 in different increasing dosages.
The different dosages of antagonists did not affect LCN2 secretion from primary rat adipocytes culture.
Could it means that the lowest concentration used (HC067 500nM and GSK219 10nM)) is adequate to occupied the receptor since there is no difference in term of band intensities when compared to the higher concentration of antagonists (HC067 1uM and GSK219 100nM)?
Is there any other possible interpretation or conclusion that can be deduce from the result?
I welcome any feedback or comment from everyone.
Thanks a lot!!
Edited by mongmetal, 30 July 2018 - 02:13 AM.