v basic question - inherited some analysis spreadheets and want to double-check....
When analysing qpcr(tho applies similarly to other techniques)
we have 4 groups
2 )diseased + control treatment
3) diseased + treatment A
4) diseased + treatment B
n=3 for each
all expression of genes already relative to housekeeper
First analysis option: One way ANOVA of group ddct values.
Second analysis, like fold-change where everything normalised to the average value of group 1)....hence we now have values for 1) of....1, 0.9, 1.1 for example....then other values for treamtent groups
For second analysis: do we one way ANOVA accross all groups, or only across the treatment groups??
Edited by erb, 15 July 2018 - 05:01 AM.