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normalisation of control samples to "1"

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#1 erb



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Posted 15 July 2018 - 05:00 AM

HI there


v basic question - inherited some analysis spreadheets and want to double-check....


When analysing qpcr(tho applies similarly to other techniques)


we have 4 groups


1)healthy control

2 )diseased + control treatment

3) diseased + treatment A

4) diseased + treatment B


n=3 for each



all expression of genes already relative to housekeeper


First analysis option: One way ANOVA of group ddct values.


Second analysis, like fold-change where everything normalised to the average value of group 1)....hence we now have values for 1) of....1, 0.9, 1.1 for example....then other values for treamtent groups



For second analysis: do we one way ANOVA accross all groups, or only across the treatment groups??





Edited by erb, 15 July 2018 - 05:01 AM.

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