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normalisation of control samples to "1"


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#1 erb

erb

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Posted 15 July 2018 - 05:00 AM


HI there

 

v basic question - inherited some analysis spreadheets and want to double-check....

 

When analysing qpcr(tho applies similarly to other techniques)

 

we have 4 groups

 

1)healthy control

2 )diseased + control treatment

3) diseased + treatment A

4) diseased + treatment B

 

n=3 for each

 

 

all expression of genes already relative to housekeeper

 

First analysis option: One way ANOVA of group ddct values.

 

Second analysis, like fold-change where everything normalised to the average value of group 1)....hence we now have values for 1) of....1, 0.9, 1.1 for example....then other values for treamtent groups

 

 

For second analysis: do we one way ANOVA accross all groups, or only across the treatment groups??

 

thanks

 

erb


Edited by erb, 15 July 2018 - 05:01 AM.





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