Hi there, I've never done a Luciferase reporter assay before and am also new to DNA cloning etc, which is why I'm very confused.
I have a plasmid with my gene promoter of interest linked to Luciferase reporter in the pGL3 basic vector, available from Addgene. I need to express it in HEK 293T cells, which are human cells. However, on looking into it further, it turns out that the only gene promoter available on Addgene is the mouse version.
I wanted to see whether my stimulus will induce transcriptional activity in my promoter (demonstrated by luciferase activity). However, having seen this is the mouse version of my promoter gene, I am wondering whether this experiment will work in human cells?
Could someone please shed some light on this? Any additional info on the assay protocol would also be useful.
Thanks so much
Edited by Natalia KM, 10 July 2018 - 12:51 AM.