Respected members and Researchers,
i am doing my experiment on SDS PAGE and 2D gel electrophoresis. Further I have to do the confirmation of my protein by western blot. In one SDS gel there are 10 samples and every lane have 4 proteins in target and my sample is about more than 100. So to perform western blot for all 4 protein for different lanes is not an easy task, As I wants expert opinion from your experiences, what would you suggest? Do I cut all the 4 proteins of my interest from every lane or can I perform all lanes together at least one gel having 10 lanes at 4 successive steps for 4 different proteins?
Thank You very much