I know this might be a stupid question, but just want confirmation either way before going through the building and validation of a few new vectors for single cell sequencing.
I am want to insert a string of A's (no polyA signal) for polyA RNA capture on a gene of interest in the middle of my lentiviral vector that also has a different promoter driving selection downstream.
Basic example lentiviral vector (expression)
I don't think this will affect the production of a full length viral transcript and packaging and might work for detecting the BC for single cell sequencing, but I am not 100% sure.
If anyone with more plasmid/lentiviral biology background can provide an answer that they believe this design would or would not work, I would greatly appreciate the input.
Thank you very much for your time and thoughts!
Edited by Beaver Aggie, 26 June 2018 - 07:50 AM.