I use the Strep-TagXT (IBA Lifesciences) purification system of my tagged protein, the elution buffer BXT has the composition of:
100 mM Tris/HCl, pH 8.0
150 mM NaCl
1 mM EDTA
50 mM biotin
Does the presence of biotin interfere with the downstream applications, for example aggregation assays? Should the buffer BXT be exchanged before proceeding?