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Biotin buffer interference after Strep-TagXT purification

Strep Tag XT BXT

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#1 Lightbulb_babies

Lightbulb_babies

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Posted 23 June 2018 - 04:00 AM

Dear all, 

I use the Strep-TagXT (IBA Lifesciences) purification system of my tagged protein, the elution buffer BXT has the composition of:

 

100 mM Tris/HCl, pH 8.0

150 mM NaCl

1 mM EDTA

50 mM biotin 

 

Does the presence of biotin interfere with the downstream applications, for example aggregation assays? Should the buffer BXT be exchanged before proceeding?

 

Thank you

 






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