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Hae III preparation for agarose gel electrophoresis

Hae III

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#1 Carollia

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Posted 22 June 2018 - 12:39 PM

My first time running this analysis. I'm going to purchase the Hae III enzyme that comes with Cut Smart buffer and gel loading dye. I haven't been able to find the instructions on how to prepare this, do I mix the content of all 3 tubes together? or do I add them separately to my PCR reaction?

 

Thanks



#2 bob1

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Posted 22 June 2018 - 12:54 PM

Restriction enzymes are used to digest DNA sequences for a variety of purposes - analysis and cloning being the two most common. What is it exactly that you are trying to do?



#3 mdfenko

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Posted 22 June 2018 - 01:08 PM

and the enzyme, buffer and dye should come with instructions (and are usually available online).

 

in general, you add buffer, dna, water and enzyme together, incubate for a period of time determined by incubation temperature, enzyme reaction speed, amount of enzyme, and amount of dna in reaction. add loading buffer to stop the reaction. load onto gel.


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