I'm a new lab coordinator and this is my first time preparing the material to run an SDS page electrophoresis analysis.
I'm adding DTT to my Laemmli buffer two months before the lab and keeping it in the freezer, is this fine?
Does 256 mg of DTT for 30 ml of Laemmli buffer sound right?
Should I autoclave all the plastic microcentrifuge vials even if they are new? what about the pipette tips?