I have been trying to set up methylight in a new lab and i failed miserably. Even the primers like ALU, COL2A1 are not working and i have tried several polymerases and even the protocols i used successfully in other labs. I made sure there is no ampurase in the polymerase etc. The primers work well with sybrgreen but when i run taqman, nothing works. Any help is greatly appreciated.
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Methylight PCR issues
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