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bands are not discrete in PCR, why?


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#1 yobou

yobou

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Posted 30 May 2018 - 05:20 PM

Dear all

I would greatly appreciate if you let me know why bands do not appear discrete after electrophoresis of PCR reaction products? they appear diffuse and broad (as in the attached file). agarose electrophoresis was done in 2% gel conc. at 50 volts (minigel) in TBE buffer.

thanks






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