My lab just received our own custom plasmid that we ordered in the form of a dry large scale prep (a small tube with just dry DNA in it). First thing i did was wrap the tube in aluminum foil and put it in the fridge.
- I need a protocol for resuspending the plasmid DNA for both use and long term storage (I believe we need TE buffer for this?).
- I need a protocol for transfection of HEK293 cells (mammalian) with our plasmid. Our cells are adherent type, growing in a media of DMEM+FBS+Glutamax.