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Help Needed Finding Some Plasmid Prep Protocols

plasmid cell culture transfection plasmid prep

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#1 Gandalf99

Gandalf99

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Posted 28 May 2018 - 08:15 AM

My lab just received our own custom plasmid that we ordered in the form of a dry large scale prep (a small tube with just dry DNA in it). First thing i did was wrap the tube in aluminum foil and put it in the fridge.

 

Protocols:

  1. I need a protocol for resuspending the plasmid DNA for both use and long term storage (I believe we need TE buffer for this?).
  2. I need a protocol for transfection of HEK293 cells (mammalian) with our plasmid. Our cells are adherent type, growing in a media of DMEM+FBS+Glutamax.






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