I would like to know your opinion about a PCR I made. I attach the image of the gel. I wanted to amplify a gene of 675 pb from a miniprep I made from a vector I subcloned. The first lane is the ladder (I used the 1kb from Thermo Fisher), the 2nd to the 10th lane are my samples, the 11th lane is the negative control without taq and the 12th lane is the negative control without DNA (I used water as the template). I see a weak band in the last lane, I don´t know if it is due to contamination because the bands of my samples look stronger and one sample did not amplify anything. What do you think it could be? Should I repeat the PCR again?
Edited by liligompac, 27 May 2018 - 01:29 PM.