My bacteria stock is at a concentration of 1x108 CFU/ml (it's in 1ml total volume). I would like to infect mice with different CFU concentrations: 5x107 5x106 5x105 5x104 each in a 40ul volume (in PBS). Could you please help me with the calculations?
1. Is this correct?
1x108 to 5x107 -> dilution factor is 2, 40ul/2= 20ul, so if I take 20ul from the 1x108 CFU/ml stock into 20ul of PBS I will get 5x107
2. When I take volume from my original stock = 1x108 CFU/ml, in order to get the new concentrations, am I changing the original 1x108 CFU/ml concentration?
Edited by kozl, 11 May 2018 - 01:33 AM.