I've been given a plasmid, and the vector map shown the antibiotic resistance gene marked 'Neo/KanR'.
Just for confirmation does it mean the gene translation products can resistant to both Neomycin and Kanamycin.
I only need to use either one antibiotic for selection purpose right?
Also, anyone know the suitable final concentration of Kanamycin to be used for selection?
Some told me 25 ug/ul, some told me 50 ug/ul. I was told too high might cause the bacteria to not able to grow at all.