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Neomycin/Kanamycin Resistant gene

neomycin kanamycin resistant gene

Best Answer bob1, 11 May 2018 - 05:59 AM

You only need to use one - generally Kan is used as it is cheaper. The Neo gene also confers antibiotic resistance for eukaryotic cells, so that you can do selection of transfected cells.

 

I would use Kan at 50 ug/ml (not ug/ul as you have above - that would make a good stock concentration though for a 1:1000 dilution to working), but if you have a low copy number plasmid then you should use at 25 ug/ml.

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#1 Thomson

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Posted 10 May 2018 - 08:34 PM

Hi, 

 

I've been given a plasmid, and the vector map shown the antibiotic resistance gene marked 'Neo/KanR'.

Just for confirmation does it mean the gene translation products can resistant to both Neomycin and Kanamycin. 

I only need to use either one antibiotic for selection purpose right?

 

Also, anyone know the suitable final concentration of Kanamycin to be used for selection?

Some told me 25 ug/ul, some told me 50 ug/ul. I was told too high might cause the bacteria to not able to grow at all.

 

Thanks



#2 bob1

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Posted 11 May 2018 - 05:59 AM   Best Answer

You only need to use one - generally Kan is used as it is cheaper. The Neo gene also confers antibiotic resistance for eukaryotic cells, so that you can do selection of transfected cells.

 

I would use Kan at 50 ug/ml (not ug/ul as you have above - that would make a good stock concentration though for a 1:1000 dilution to working), but if you have a low copy number plasmid then you should use at 25 ug/ml.



#3 Thomson

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Posted 13 May 2018 - 05:13 PM

Thanks. Sorry for the typo. I'd try with 50ug/ml final concentration. ;)







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