1 reply to this topic

[yobou]

dear all

I would greatly appreciate if you help me know how to predict the amplicon size in conventional PCR using the sequences of forward and reverse primers? is there a website that can provide such info?

here are the sequences of alpha-tubulin primers used:

forward: ACACCTTCTTCAGTGAGACAGG

reverse: CTCATTGTCTACCATGAAGGCAC

thanks

[bob1]

Try the primer3 site - you can put in the sequence of your gene and add the primer sequences, so I should show you where these match.

 

Otherwise you can align them to the sequence using any number of alignment programs and note the places they bind.