Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

RNA isolation RBC RPE buffer swap

rbc rpe ffpe rneasy

  • Please log in to reply
1 reply to this topic

#1 Sandra1

Sandra1

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 03 May 2018 - 04:50 AM

Hi,

 

Does anyone know if it is possible to continue isolation when you have first used RPE buffer (washing RNA) in stead of RBC buffer (binding RNA to column) on the columns?

Is there any way to save the RNA?

Help is needed!

 

Sandra

 



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,540 posts
548
Excellent

Posted 04 May 2018 - 06:21 AM

I'm not 100% sure, but I think you have just lost your RNA - the binding buffer makes it a particular pH which allows the RNA to bind to the column. The washing buffer is largely ethanol and will precipitate the RNA (so it doesn't get washed off the column after binding), but won't let it bind if the RNA is not already bound. 







Also tagged with one or more of these keywords: rbc, rpe, ffpe rneasy

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.