I use a very specialised protocol called DNA combing which demands an anti-CldU and an anti-IdU antibody that do not cross-react. The field has always used an anti-CldU rat antibody from clone BU1/75: the only verified antibody that does not cross-react on IdU in this application. Unfortunately, not all commercial sources are the same! It can currently be bought from Abcam and Novus, but both sources cross-react on IdU. Sources that did NOT were SeraLab BU1/75, Cat. MA5 250, AbD Serotec Cat. OBT 0030, and Thermoscientific (made by Invitrogen) Cat. MA-1-82088. ALL these have been sadly discontinued! This puts work using the DNA combing technique in my lab and others into significant jeopardy. I am appealing to any scientists who may have the antibodies listed above stored away in their freezers...
Submit your paper to J Biol Methods today!
Does anyone have these (sadly-discontinued) anti-BrdU antibodies in a freezer?
Started by cmerrick, Apr 25 2018 03:46 AM
dna replication antibody BrdU DNA combing immunofluorescence
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Also tagged with one or more of these keywords: dna replication, antibody, BrdU, DNA combing, immunofluorescence
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